Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method
Maintenance of Culture of Test Organisms 8. Safety Precautions reasonable precaution must be aatc to eliminate this risk to the laboratory personnel and to personnel in the associated environment.
May be sterilized in 1, mL borosilicate glass flasks and petri dishes poured from this. A standard strain of bacteria is used which is specific to the requirements of the material under test.
Mehod, when the intent is to demonstrate bacteriostatic activity by the diffusion of the antibacterial agent through agar, Method fulfills this need. Allow methd to gel firmly before inoculating. Foreword The Parallel Streak Method has filled a need for a relatively quick and easily executed qualitative method to determine antibacterial activity of diffusable antimicrobial agents on treated textile materials. A zone of inhibition occurs as a result of the diffusion of an antimicrobial agent from the specimen.
The results of using this procedure have been demonstrated by Committee RA31 to be reproducible mehtod various laboratories working with materials containing residual amounts of antibacterial agents as determined by chemical assay after multiple standard washings. Check culture purity by making streak plates periodically and observing for a single species-characteristic type of colonies.
They may be any convenient size. Leonard, Technical Director, at the address shown below. At the conclusion of two weeks, make a fresh transplant from stock culture.
After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen. Users of this test method are expressly advised that determination of the validity of any such patent rights, and methid risk of infringement of such rights, are entirely their own responsibility. These safety precautions are for information purposes only.
Wear protective clothing and respiratory protection that prevents penetration by the bacteria. Mix well using appropriate teet. In the Parallel Streak Method, the agar surface is inoculated making it easier to distinguish between the test organism and contaminant organisms which may be present on the unsterilized specimen.
The test is followed in the cases where the antibacterial agent is migratory i. The hard copy print may only be distributed to other employees for their internal use within your organization. AATCC grants you a license as follows: Purpose and Scope cluding corresponding untreated controls of the same material, are placed in intimate contact with nutrient agar see 7. Notes and References Antibacterial Aactc Assessment of Textile Materials: Peptone Bacto-peptone see The Parallel Streak Method has proven effective over a number of years of use in providing evidence of antibacterial activity against both Gram positive and Gram negative bacteria.
AATCC Antimicrobial Testing Lab – Accugen Laboratories, Inc.
Avoid mutation by strict adherence to monthly stock transfers. Other recommended strains are listed below in Section 6.
AATCC Test Method Limitations The method is not suitable for materials which are not migratory and are unable to diffuse thru the bacterial growth medium. The precautions are ancillary to the testing procedures and are not intended to be all inclusive. Wear safety glasses in all laboratory areas. The test Method determines antibacterial activity of diffusable antimicrobial agents on treated awtcc materials. This is not necessary if prepared, dehydrated medium is used.
Parallel Streak Method Developed in by AATCC Committee RA31; reaffirmed, ; editorially revised,; revisedwith title change; editorially revised and reaffirmed After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen.
In addition, the electronic file may not be distributed elsewhere over computer networks or otherwise. All OSHA standards and rules must also be consulted and followed. BoxManassas VA ; tel: AATCC MethodAntibacterial Finishes on Textile Materials, Assessment of, is a quantitative procedure which is adequately sensitive but is cumbersome and time consuming for routine quality control and screening tests. Rectangular specimens cut 25 – 50 mm They can be any convenient size.
AATCC 147 – Antimicrobial Fabric Test
This may be accomplished more easily by pressing the specimen to methov agar surface with a biological section lifter or with a spatula which has been sterilized by flaming and then air cooled immediately before use.
Report of results will include an observation of zones of inhibition and growth under the specimen if present. Department of Health and Human services publication Biosafety in Microbiological and Biomedical Laboratories should be consulted see A 50 TM Copyright? Culture Medium mm length permits the specimens to lie across 5 parallel inoculum streaks each of diminishing width from about 8 mm to 4 mm wide.
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