Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. It has not previously been reported. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and. Proc Natl Acad Sci U S A Sep 29; Draft Genome Sequence of Actinomadura madurae LIID-AJ, Isolated from a Human Mycetoma Case. Vera-Cabrera L.

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A systematic approach for discovering novel, clinically relevant bacteria. A template procedure for Ion Torrent sequencing was performed using a OneTouch 2 system Life Technologiesand sequencing was performed masurae an Ion PGM sequencer Life Technologies using a bp sequencing kit and a v2 chip according to the manufacturer’s instructions. Next-generation sequencing in clinical microbiology.

QD was resumed for an 8-week course, but no response in the amount of discharge or reduction in the size of the nodules was achieved and the antibiotic was consequently discontinued. Int J Syst Evol Microbiol. Mycetoma in South India: Departments of Laboratory Medicine a. Please review our privacy policy.

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Approximately 4 years prior to the current presentation, the diagnosis of actinomycotic mycetoma was reportedly established by histology, and she was initiated on antibiotic therapy with penicillin mg orally [p. Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions. Actimomadura in a separate window. Salipantea Dhruba J.


Rapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections. Given the extent of infection, neither additional antibiotic therapy nor debridement with subsequent reconstruction was felt to be a viable option, and a recommendation was made for below-the-knee amputation.

Nonmycetomic Actinomadura madurae infection in a patient with AIDS.

Journal List J Clin Microbiol v. In this case report, we have utilized the approach to investigate the causative agent of biopsy-proven actinomycotic mycetoma. Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial specimens. Int J Syst Bacteriol.

Actinomadura madurae – Global Catalogue of Microorganisms

Comparison of species distribution and antimicrobial susceptibility of madurwe actinomycetes from clinical specimens. Footnotes Published ahead of print 9 October Prospects for the future using genomics and proteomics in clinical microbiology.

No new lesions evolved while she was on this therapy, but approximately 1 year prior to presentation the patient self-discontinued ciprofloxacin and shortly thereafter noted increasing drainage from her foot.


PCR products were purified using 0. Culture of discharged material reportedly returned with overgrowth of skin flora and provided no evidence of infection with Actinomyces species. Comparison of DNA extraction methods for microbial community profiling with an application to pediatric bronchoalveolar lavage samples.

Actinomadura – Wikipedia

National Center for Biotechnology InformationU. A portion of the specimen actinomzdura concurrently submitted for culture in order to establish the identity of the aerobic actinomycetes-like organism and to evaluate its antibiotic sensitivities; however, the culture quickly became overgrown by Staphylococcus aureuspreventing culture for slower-growing aerobic actinomycetes species.

Barbeec, d Christopher Rosenthala Brad T. She reported a long course of originally pruritic lesions on the plantar instep of her right foot which over many years developed into multiple bulbous nodules with central pinpoint ulcerations expressing purulent and granular material. This actinomafura is one of the classical agents of actinomycotic mycetoma and is consistent with the organism visualized histologically.

BIDand doxycycline mg p. QIDciprofloxacin mg p. Address correspondence to Stephen J. Actinomadura Lechevalier and Lechevalier